Students

Selected Senior Theses

Effects of PerC regulation and membrane vesicles of enteropathogenic Escherichia coli on a pro-inflammatory response
By Vikram Chan-Herur, May 2017

Distribution of the Transcriptional Regulator Ler in E. coli Using PALM
By Kristin Hirata, May 2017

A tale of two phages: Investigation of the therapeutic potential of SiphoRed1 and SiphoRed2
By Madeline Dinsdale, May 2017

Cohabitation to Restore the Rat Microbiome after Antibiotic-Induced Dysbiosis
By Yasemin Lopez, May 2017

The Age of Phage
By Leah Cepko, May 2016

Regulation of anaerobic respiration by PerC in enteropathogenic Escherichia coli
By Amy Platenkamp, May 2016

The Role of Hfq in the Reduction of Virulence in EPEC by Zinc
By Maggie Cheney, May 2015

Whole-Transcriptome Analysis of the PerC Regulon Identifies Novel Niche-Adaptive Mechanisms in enteropathogenic Escherichia coli
By Jossef Osborn, May 2015

Altering Ler Linker Hydrophobicity Diminishes Regulatory Activity
By Jules Weinstein, May 2015

PerC Enhances Oxidative Stress Response in Enteropathogenic Escherichia coli
By Karen Dewey, May 2014

Using Bacteriophages to Clear Bacterial Biofilms
By Jennifer Farrell, May 2014

Understanding the Mechanism of ler Regulation by PerC
By Gus Kilgore, May 2014

Hydrophobicity of the Ler Linker is Necessary for Transcriptional Activation
By Abe Leiser, May 2014

The Sigma E-Dependent Pathway of Enteropathogenic Escherichia coli Mediates Inhibition of LEE Virulence by Zinc
By Yuan Xue (Soso), May 2014

Zinc as a Therapeutic Agent in Enteric Infections
By Anand Panchal, May 2013

Importance of Ler Linker Region in Oligomerization and E. coli Pathogenesis
By Shaurav Regmi, May 2013

Metabolic niche: the yqeF gene of enteropathogenic Escherichia coli 
By Rachel Strominger, May 2013

Mechanism and Importance of Ler Oligomerization in E. coli Pathogenesis
By Gabriel Butterfield, May 2012

Characterization of yqeF, a Gene Upregulated by the Virulence Regulator PerC of Enteropathogenic Escherichia coli
By Rosie Lawrence-Pine, May 2011

Microbial Diversity on the Anode Surface of a Reed Lake Sediment Fuel Cell
By Charles Morse, May 2011

Characterization and Mechanisms of Zinc Acetate Mediated Downregulation of Virulence Genes in Enteropathogenic Escherichia coli
By Katherine Thomas, May 2011

Are the N-Terminal Residues 4-47 of Ler Sufficient for Dimer Formation?
By Emily Lorenzen, May 2010

CfaD: A Global Regulator of Enterotoxigenic Escherichia coli Virulence in the Nematode Caenorhabditis elegans
By Gabriel Holt, May 2010

Sixteen Fragments: A Structural Study
By David Mavor, May 2009

Ingestion by Caenorhabditis elegans Provides Protection Against Chemical Treatment to Enterotoxigenic Escherichia coli
By Daniel Glaser, May 2009

Regulation of Virulence in Enteropathogenic Escherichia coli: In Search of a Consensus Binding Sequence for Ler
By Jeannette L. Tenthorey, May 2009

A Nematode Model for an Inconvenient and Deadly Disease: Using Caenorhabditis elegans to Investigate the Pathogenesis of Enterotoxigenic Escherichia coli
By Emily Warschefsky, May 2009

Methane production in response to sulfate concentration in batch anaerobic digesters
By Laura E. Mulshine, May 2008

An investigation of two similar but different proteins in enteropathogenic Escherichia coli: Ler and H-NS
By Melissa Zarr, May 2008

Creation of a proU::LEE5 hybrid gene to elucidate the regulatory mechanism of Ler in Enteropathogenic Escherichia coli
by Trevor Young, May 2007

The CfaR Positive Regulator of Colonization Factor Antigen I (CFA/I) Pili is Important for Enteropathogenic Escherichia coli Infection of the Nematode Caenorhabditis elegans
by Rebecca J. Kreston, May 2007

Understanding Virulence: in vitro Analysis of H-NS and Ler Mediated
Regulation of the LEE5 Regulatory Region in Enteropathogenic Esherichia
coli
.
by Anna M. Carmona, May 2006

Regulation of Enteropathogenic Escherichia coli Virulence Genes by the SOS Response
by Derek C. Galligan, May 2005

The Enteropathogenic Escherichia coli global regulator Ler is critical to pathogenesis in the nematode Caenorhabditis elegans
by Alex M. Barron, May 2005

Characterization of the C-terminal Binding Domain of Ler
by Katharine L. Horback, May 2004.

Colonization of Caenorhabditis elegans by Enteropathogenic E. coli
by Andrew S. Korson, May 2004

Reversible Frameshift mutations in the perA gene of Escherichia coli
by Rebecca S. Bart, May 2003

Interactions of Environmental and Transcriptional Factors in Regulation of Enteropathogenic Escherichia coli Virulence Genes
by Alhaji Ceesay, May 2003

Sucrose Utilization in Enteropathogenic E. coli
by Kristin Harper, May 2002

Ler and H-NS Medited Transcriptional Regulation of the LEE5 Operon of Enteropathogenic E. coli
by Jonathan W. Fowlkes, December 2002

Ler Mediated Regulation of the Tir Operon in Enteropathogenic E. coli by Footprinting and Deletion Analysis
by Kristie J. Miller, May 2001

Sucrose Metabolism in Pathogenic E. coli
by Leslie A. Hoover, May 2001

Presentations

Hirata, K, Zhang , Y, Nan, X and J Mellies. Ler’s In Vivo Oligomerization in Pathogenic Escherichia coli. Presented General Meeting of the American Society for Microbiology, New Orleans, June 1-5, 2017.

Cepko, L, Garling, E, and J Mellies. The Age of Phage in Portlandia: Allopatric Isolation and Genomic Characterization of Siphoviridae Phage that kill E.coli pathotypes. Presented at the Molecular Genetics of Bacteria and Phages Meeting, University of Wisconsin, Madison, August 8-12, 2016.

Mellies, J, Platenkamp, A, Ben-Avi, L, and J Osborn. PerC Manipulates Metabolism and Surface Antigens in Enteropathogenic Escherichia coli. Presented at the Molecular Genetics of Bacteria and Phages Meeting, University of Wisconsin, Madison, August 8-12, 2016.

Weinstein, J, Yan, J, and JL Mellies. I48R substitution in the Ler linker of enteropathogenic Escherichia coli diminishes in vivo activity. Presented General Meeting of the American Society for Microbiology, New Orleans, May 30 – June 2, 2015.

Xue, Y, Osborn, J and JL Mellies. Molecular mechanism of zinc disruption of Enteropathogenic Escherichia coli pathogenesis. Presented at The Microbial Toxins & Pathogenicity Gordon Research Conference, Waterville Valley, NH, July 20-25, 2014.

Mellies, JL, Lawrence-Pine, R, Gilbert, M, and R Strominger. The plasmid-encoded regulator PerC stimulates central metabolism in enteropathogenic E. coli. Presented at the General Meeting of the American Society for Microbiology, Denver, May 18-21, 2013.

Lawrence-Pine, R and J Mellies. Over-expression of YqeF, a putative acetyl-CoA acetyltransferase, causes auto-aggregation in enteropathogenic Escherichia coli. Presented at the Molecular Genetics of Bacteria and Phage Meeting, Madison, WI, August 2-7, 2011.

Turvey, M, Thomas, K, Crane, J, Boedecker, E, and J Mellies. The Regulatory Network Controlling Zinc Inhibition of the LEE of Enteropathogenic Escherichia coli. Presented at the General Meeting of the American Society for Microbiology, New Orleans, Louisiana, May 21-24, 2011.

Mellies, JL, Boniface, C, Lorenzen, E and G Benison. Structural Analysis of the N-Terminal and C-Terminal Domains of Ler in Enteropathogenic Escherichia coli. Presented at the General Meeting of the American Society for Microbiology, San Diego, California, May 23-27, 2010.

Mellies, J, Tenthorey, J, Mavor, D, Boniface, C, and J Connell. Molecular features of Ler that distinguish the protein from H-NS. Presented at the Molecular Genetics of Bacteria and Phage Meeting, Madison, WI, August 4-9, 2009.

Mellies, JL, Larabee, F, Fowlkes, J, Horback, K, and M Zarr. The Ler Linker Domain is Necessary for Anti-Silencing Activity in Enteropathogenic Escherichia coli. Presented at the General Meeting of the American Society for Microbiology, Boston, June 1-5, 2008.

Mellies, JL, Larabee, F, Haack, K, and D Mavor. The Ler Linker Domain Is Essential for Anti-Silencing Activity in Enteropathogenic Escherichia coli. Presented at the Molecular Genetics of Bacteria and Phage Meeting,
Madison, WI, August 7-12, 2007.

Kreston, RJ, Barron, MS and JL Mellies. Using the Nematode Caenorhabditis elegans to Model Enterotoxigenic Escherichia coli Infection. Presented at the General Meeting of the American Society for Microbiology, Toronto, Canada, May 20-25, 2007.

Mellies, JL, Haack, K and DC Galligan. SOS Regulation of the Type III Secretion System of Enteropathogenic Escherichia coli. Presented at the Gordon Research Conference on Microbial Stress Response, Mt. Holyoke College, South Hadley, Massachusetts, July 9-13, 2006.

Carmona, A.M., Glasfeld, A., and JL Mellies. Molecular Mechanism of LEE5 Transcription in Enteropathogenic Escherichia coli: in vitro Analysis of H-NS and Ler Binding. Presented at the Northwest Branch Meeting of the American Society for Microbiology, Seattle, Washington, March 10-12, 2006.

Barron, A, Haack, K, and JL Mellies. Caenorhabditis elegans as a Model for Enteropathogenic Escherichia coli Infection. Presented at the General Meeting of the American Society for Microbiology, Atlanta, Georgia, June 5–9, 2005.

Galligan, D, Haack, K, and JL Mellies. Regulation of EPEC Virulence Genes by DNA Damage Signaling. Presented at the General Meeting of the American Society for Microbiology, Atlanta, Georgia, June 5–9, 2005.

Barron, A, Galligan, D , and JL Mellies. Caenorhabditis elegans as a Model Host for Enteropathogenic Escherichia coli (EPEC) Pathogenesis. Presented at the Northwest Branch Meeting of the American Society for Microbiology, Vancouver, British Columbia, Canada, August 8-10, 2003.

Mellies, JL, Ceesay, A, Fowlkes, J, and Haack, K. Ler and H-NS Mediated Regulation of the LEE5 (tir) Operon in Enteropathogenic E. coli. Presented at the General Meeting of the American Society for Microbiology, Washington, D.C., May 18-22, 2003.

Harper, KN and L Hoover, K Haack, I Okeke and JL Mellies. Sucrose Utilization in Enteropathogenic E. coli. Presented at the General Meeting of the American Society for Microbiology, Salt Lake City, Utah, May 19-23, 2002

Mellies, JL, CL Robinson, K Haack, K Miller, and K Harper. Mechanism of Ler-Mediated Activation of tir in Enteropathogenic E. coli. Presented at the General Meeting of the American Society for Microbiology, Salt Lake City, Utah, May 19-23, 2002.

Awards

Jossef Osborn ('15) 
The Reed President's Winter Fellowship to visit the South African Tuberculosis Vaccine Initiative, 2014-2015

Rosie Lawrence-Pine ('11)
ASM Undergraduate Research Fellowship, 2010-2011

Jeannette Tenthorey ('09)
NSF Graduate Fellowship, 2012

Anna Carmona ('06)
Best Undergraduate Research Poster Presentation at the Northwest Branch
Meeting of the American Society for Microbiology, Seattle, Washington,
March 10-12, 2006.

Alex Barron ('05)
Student Travel Award - ASM General Meeting, Atlanta, Georgia, June 2005

Derek Galligan ('05)
ASM Undergraduate Research Fellowship, 2004-2005

Kristin Harper ('02)
Student Travel Award - ASM General Meeting, Salt Lake City, Utah, May 2002
NSF Graduate Fellowship ('03) and Howard Hughes Medical Institute Pre-Doctoral Fellowship ('03)

Leslie Hoover ('01)
NIH Post-Baccalaureate Research Fellowship, Bethesda, Maryland, 2001-2003

Beyond Thesis

Thesis students from my lab have gone on to the following:

Graduate Programs
Emory University, University of California at Davis, University of California, Berkeley, University of Michigan, Johns Hopkins University, University of California, San Francisco, Dartmouth College, Rockefeller University, University of Pennsylvania, Harvard University, and Stanford University.

Medical Schools
University of Maryland School of MedicineTulane University School of Public HealthGeorge Washington University School of MedicineOregon Health & Science UniversityUniversity of Arizona College of Medicine, The University of Oklahoma, and Albert Einstein College of Medicine.

Biotechnology
MedImmune, Inc., Silver Lake Research Corporation and Biovest International.

Law School
Santa Clara University, Duke University School of Law.

Public Health
Tulane University School of Public Health and Tropical Medicine and University of California, Berkeley, School of Public Health.

Veterinary Schools
Tufts University and Massey University