Course Syllabus

Week

Date

Lecture

Readings

 

 

I. Microbial Cell Biology

1

1-23

It's a Microbial World!

Prescott Ch. 1

1-25

Flagellar Motility as a Biological Paradigm

Macnab (1999) The bacterial flagellum: reversible rotary propellor and type III export apparatus. Journal of Bacteriology 181: 7149-7153.

1-27

Prokaryotic Cell Structure and Function I

Prescott Ch. 3

2

1-30

Prokaryotic Cell Structure and Function II

2-1

Nutrition

Prescott Ch. 6

2-3

Growth

Prescott Ch. 7

3

2-6

Control of Microbial Growth

Prescott Ch. 8

2-8

Microbial Diversity

Prescott Ch. 17.
Kroes et al. (1999) Bacterial Diversity within the human subgingival crevice. Proceedings of the National Academy of Sciences USA 96: 14547-14552.

2-10

Metabolism I

Prescott Ch. 9 pages 208-222.
Prescott Ch. 10 pages 228-254.

Schultz et al. (1999) Dense populations of a giant sulfur bacterium in Namibian shelf sediments. Science 284: 493-495.

4

2-13

Metabolism II

 

II. Microbial Genetics

2-15

DNA Replication

Prescott Ch. 12 pages 297-305

2-17

DNA Repair / Mutation

Prescott Ch. 14 pages 363-376.
Oliver et al. (2000) High frequency of hypermutablePseudomonas aeruginosa in cystic fibrosis lung infection.Science 288: 1251-1253.

5

2-20

Recombinant DNA

Prescott Ch. 15

 

 

Prescott Ch. 14 pages 376-396.

 

Lawrence and Ochman (1998) Molecular archaeology of the E. coli genome. Proceedings of the National Academy of Sciences USA 95: 9413-9417.

2-22

MIDTERM EXAM I

2-24

Horizontal Gene Transfer I

6

2-27

Horizontal Gene Transfer II

3-1

Transcription

Prescott Ch. 13 pages 331-342

3-3

Sensing the Environment: Two-Component Systems I

Prescott Ch. 13 pages 346-354.

7

3-6

Sensing the Environment: Two-Component Systems II

Shukla et al. (1998) Flagellar motor-switch binding face of CheY and the biochemical basis of suppression by CheY mutants that compensate for motor-switch defects in Escherichia coliThe Journal of Biological Chemistry 273: 23993-23999.

3-8

Quorum Sensing

Prescott Ch. 13 pages 355-356.
Bassler (1999) How bacteria talk to each other: regulation of gene expression by quorum sensing. Current Opinion in Microbiology2: 582-587.

3-10

Guest Lecturer JJ Miranda, PhD

Serfling et al. (1985) Eukaryotic Transcriptional Control, TIG 1, 224-230.

 

 

SPRING BREAK

8

3-20

Regulation of Transcription: The Ler Story

Haack et al. (2003) Interaction of Ler at the LEE5 (tir) operon of EPEC. Infection and Immunity 71: 384-392.

 

III. Interactions of Microbes and Hosts 

3-22

Introduction to Infectious Disease

Prescott Ch. 31

3-24

Brazilian Purpuric Fever- A Case Study

Li et al. (2003) Identification and characterization of genomic loci unique to the Brazilian purpuric fever clonal group of H. influenzae biogroup aegyptius: functionality explored using meningococcal homology Molecular Microbiology 47: 1101-1111.

9

3-27

Secretion I

Galán and Wolf-Watz (2006) Protein delivery into eukaryotic cells by type III secretion machines. Nature 444: 567-573.

3-29

Secretion II

Feldman et al. (2002) SycE allows secretion of YopE-DHFR hybrids by the Yersinia enterocolitica type III Ysc system. Molecular Microbiology 46: 1183-1197.

3-31

Biofilms

Prescott Ch. 7 pages 183-186.

Flemming et al. (2016) Biofilms: an emergent form of bacterial life. Nature Reviews Microbiology 14: 563-575.

10

4-3

E. coli O157

 Lim et al. (2013) A brief overview of Escherichia coli O157:H7 and its plasmid O157 Journal of Microbiology and Biotechnology 20: 5–14.

4-5

MIDTERM EXAM II

 

4-7

Vibrio cholerae

Nelson et al. (2009) Cholera transmission: the host, pathogen and bacteriophage dynamic. Nature Reviews Microbiolog7, 693-702

11

4-10

Multi-Drug Resistance

Prescott Ch. 34 pages 843-847.
Ling et al. (2014) A new antibiotic kills pathogens without detectable resistance. Nature 517: 455–459.

4-12

The Microbiome

Prescott Ch. 30 pages 731-736.

Cho et al. (2012) Antibiotics in early life alter the murine colonic microbiome and adiposity. Nature 488: 621-629.

4-14

Viruses: Introduction

Prescott Ch. 5

12

4-17

Bacteriophage I

Prescott Ch. 5

4-19

Bacteriophage II

4-21

FILM: The Virus That Cures

 

 

Prescott Ch. 37 pages 899-903.

13

4-24

Influenza I

4-26

Influenza II

Webby and Webster (2003) Are we ready for pandemic influenza? Science 302: 1519-1522.

4-28

Epidemiology

Prescott Ch. 36

 

5-11

FINAL EXAM 1 pm – 4 pm; Psych 105